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pu6 cj e sgrna  (Addgene inc)


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    Structured Review

    Addgene inc pu6 cj e sgrna
    Pu6 Cj E Sgrna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pu6+cj+sgrna/pU6-Fn-crRNA+(Plasmid+%2378958)/pmc12812445-326-44-46
    Average 93 stars, based on 3 article reviews
    pu6 cj e sgrna - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Construct:

    Article Title: High-efficiency base editing for nuclear and mitochondrial DNA with an optimized DYW-like deaminase.
    Article Snippet: The amplicons were then cloned into the wildtype SsCBE-UGI-C2 vector using Gibson Assembly Master Mix (New England Biolabs). .. The cjSsCBE2 was constructed by exchanging the APOBEC1 domain of cjCBEmax with the SsdAtoxSRE domain, and the pAAV-cjABE8e-gRNA-ANGPT2-HPD-2 was modified to construct the single AAV vector encoding cjSsCBE2.25 The gRNAs were constructed using the pRG2Z vector (Addgene, catalog no. 104174) and pU6-Cj-sgRNA (single-guide RNA; Addgene, catalog no. 89753). ..

    Article Title: Targeted dual base editing with Campylobacter jejuni Cas9 by a single AAV-mediated delivery
    Article Snippet: .. Materials And Methods Plasmids constructs pRGEN-CMV-CjCas9 (#89752; addgene, Watertown, MA, USA) was used for pCMV-cjCas9 transfection and pU6-Cj-sgRNA (addgene #89753) was used to generate the gRNAs of cjCas9. ..

    Article Title: Efficient DNA base editing via an optimized DYW-like deaminase
    Article Snippet: .. The gRNAs were constructed using pRG2Z vector (Addgene #104174) and pU6-Cj-sgRNA (Addgene #89753). .. HEK293T/17 (ATCC CRL–11268) and HeLa (ATCC CCL–2) were maintained in DMEM medium with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin.

    Modification:

    Article Title: High-efficiency base editing for nuclear and mitochondrial DNA with an optimized DYW-like deaminase.
    Article Snippet: The amplicons were then cloned into the wildtype SsCBE-UGI-C2 vector using Gibson Assembly Master Mix (New England Biolabs). .. The cjSsCBE2 was constructed by exchanging the APOBEC1 domain of cjCBEmax with the SsdAtoxSRE domain, and the pAAV-cjABE8e-gRNA-ANGPT2-HPD-2 was modified to construct the single AAV vector encoding cjSsCBE2.25 The gRNAs were constructed using the pRG2Z vector (Addgene, catalog no. 104174) and pU6-Cj-sgRNA (single-guide RNA; Addgene, catalog no. 89753). ..

    Bioprocessing:

    Article Title: High-efficiency base editing for nuclear and mitochondrial DNA with an optimized DYW-like deaminase.
    Article Snippet: The amplicons were then cloned into the wildtype SsCBE-UGI-C2 vector using Gibson Assembly Master Mix (New England Biolabs). .. The cjSsCBE2 was constructed by exchanging the APOBEC1 domain of cjCBEmax with the SsdAtoxSRE domain, and the pAAV-cjABE8e-gRNA-ANGPT2-HPD-2 was modified to construct the single AAV vector encoding cjSsCBE2.25 The gRNAs were constructed using the pRG2Z vector (Addgene, catalog no. 104174) and pU6-Cj-sgRNA (single-guide RNA; Addgene, catalog no. 89753). ..

    Plasmid Preparation:

    Article Title: High-efficiency base editing for nuclear and mitochondrial DNA with an optimized DYW-like deaminase.
    Article Snippet: The amplicons were then cloned into the wildtype SsCBE-UGI-C2 vector using Gibson Assembly Master Mix (New England Biolabs). .. The cjSsCBE2 was constructed by exchanging the APOBEC1 domain of cjCBEmax with the SsdAtoxSRE domain, and the pAAV-cjABE8e-gRNA-ANGPT2-HPD-2 was modified to construct the single AAV vector encoding cjSsCBE2.25 The gRNAs were constructed using the pRG2Z vector (Addgene, catalog no. 104174) and pU6-Cj-sgRNA (single-guide RNA; Addgene, catalog no. 89753). ..

    Article Title: Efficient DNA base editing via an optimized DYW-like deaminase
    Article Snippet: .. The gRNAs were constructed using pRG2Z vector (Addgene #104174) and pU6-Cj-sgRNA (Addgene #89753). .. HEK293T/17 (ATCC CRL–11268) and HeLa (ATCC CCL–2) were maintained in DMEM medium with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin.

    Transfection:

    Article Title: Targeted dual base editing with Campylobacter jejuni Cas9 by a single AAV-mediated delivery
    Article Snippet: .. Materials And Methods Plasmids constructs pRGEN-CMV-CjCas9 (#89752; addgene, Watertown, MA, USA) was used for pCMV-cjCas9 transfection and pU6-Cj-sgRNA (addgene #89753) was used to generate the gRNAs of cjCas9. ..

    Article Title: Targeted dual base editing with Campylobacter jejuni Cas9 by single AAV-mediated delivery
    Article Snippet: .. pRGEN-CMV-CjCas9 (#89752; Addgene, Watertown, MA, USA) was used for pCMV-cjCas9 transfection, and pU6-Cj-sgRNA (Addgene #89753) was used to generate the gRNAs of cjCas9. ..



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